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"Shania Twain Is Officially Divorced and 'Radiantly Happy'"

Standing outside the AT&T building in downtown Jacksonville on a chilly afternoon last week, she said she wouldn't quit until the price passes $5 a pack

Her healthy-looking hair appeared to be freshly blown out in loose waves, as she carried her large black shoulder bag and strolled down the street

The upstream and downstream fragments were linked by overlapping PCR, digested by restriction enzymes XbaI and SphI (Takara, Shiga, Japan), and then ligated into the PK18 vector (PK18-U-D) by T4 DNA ligase enzyme (Takara, Shiga, Japan) and transformed into R