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Patch pipettes (48 M) were pulled from thin wall borosilicate glass (G150TF-3, Warner Instruments) using a micropipette puller (P-87, Sutter Instruments, Novato, CA) and filled with a KGlu-based intra-pipette solution containing (in mM): 116 K-gluconate, 10-20 HEPES, 0.5 EGTA, 6 KCl, 2 NaCl, 4 ATP, 0.3 GTP, and 2 mg/mL biocytin (pH adjusted to 7.2)

Here are some guidelines: 1

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This process, known as autoinoculation, can lead to sores appearing in places like the eyes or fingers, which is why hygiene during outbreaks is so important