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Some minor wear to the engine turned engraving on the back but nothing serious

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(Note: international terminology is evolving toward metabolic dysfunction-associated steatotic liver disease [MASLD], though NAFLD and NASH remain widely used in UK clinical practice.) Alcohol-related fatty liver disease develops through excessive alcohol consumption, which directly damages liver cells and disrupts fat metabolism

As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig