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Microvessel isolation was carried out as described by our lab (Bernstein et al., 2019) with modifications suggested by Lee and colleagues (2019)

In fact, it probably gave Fruit Stripe Gum a run for its money at the time

Smoking tobacco will have been cut or otherwise split, twisted or pressed into blocks which can be smoked without further industrial processing

They permit different levels of study: organ, tissue, cell (one or several populations), they allow large screening of effects with a very small amount of test material and are very well adapted for the study of mechanisms, mainly for short term studies [17, 20, 21]