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doi: 10.1152/ajpendo.00053.2007

The quantitation of peptides using isotopic labeling is performed based on the intensity of peptides measured in MS1 spectra, while that of the isobaric tagging approach is carried out based on the intensity of tags cleaved from labeled peptides in MS2 spectra with signature masses (e.g., 126, 127, 128, 129, 130, and 131 m/z)

After digestion with BamHI and SalI, the DNA fragment was cloned into pQE-30 Xa

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