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Briefly, cells grown on 12 mm glass coverslips (Matsunami Glass Industry, Osaka, Japan, Cat# 1-S) coated with 0.2% gelatin from porcine skin (Sigma Aldrich, Cat# G2500) were incubated with 75 nM LysoTracker Red for 30 min at 37 C in the dark

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But did the perpetually camera-shy Benning ever consider including himself, as a kind of self-portrait

It was a dangerous combination, one that made her want to sit back and let the hours speed by while her body stewed in its own thoughts