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Because popcorn lung causes scarring in the lungs, it can have a range of effects on the body, including: Shortness of breath Short, dry cough Wheezing (without the presence of asthma or a cold) Reduced tolerance for physical activities Fatigue Clearly, popcorn lung is not the only cause of these symptoms, which are extremely uncommon

The thermal cycle conditions of PCR amplification were as follows:94C for 3 min, 45 cycles, 94C for 20 s, 57C for 25 s, 72C for 45 s, and finally 72C for 10 min

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