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While there are different models to study glutathione metabolism, in this study we used BSO as it is a specific and potent chemical inhibitor of the rate limiting enzyme GCLC (38)

Therefore, low-dose chelation strategies are preferred to maintain neuroprotection without disrupting systemic iron balance

Screening by determining total homocysteine (tHcy) in dried blood spots is only occasionally performed given the need for a reduction step prior to LC-MS/MS assay and associated costs [42]