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An assay that lacks sensitivity at low cell numbers or introduces variability between plates, will undermine every downstream result

[7] [8] NAD+ and NADH are continuously recycled during redox reactions, but during other metabolic activities, they are absorbed by NAD+-dependent enzymes and may subsequently become depleted over time, increasing the risk of DNA damage, age-related illnesses, and mitochondrial malfunction

This provides a biochemical mechanism for the VD-deficiency and potential benefits of GSH treatment in reducing 25(OH)VD 3 -deficiency

It's a period of intense brain remodeling (see CBD and teen anxiety or CBD and child anxiety)