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SOD activity was measured by an adaptation of the method of McCord & Fridovich ( Reference McCord and Fridovich 34 ) .The xanthine/xanthine oxidase system was used to generate the superoxide anion

The samples underwent a series of procedures for preparation, including dehydration in alcohol, clarifying in xylene, synthetic wax infiltration, and blocking out into Paraplast tissue embedding media

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In obesity and metabolic dysfunction, NNMT activity increases substantially, accelerating NAD+ depletion and metabolic decline