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The review documented GSHs broad roles including antioxidant defence, nutrient metabolism, gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transduction, cytokine production, immune response, and protein glutathionylation

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For callose deposition in root, roots were stained with 1% (w/v) aniline blue, then washed in Sorensens phosphate buffer and observed at light intensity (5 M photons m 2 s 1 ) at 358 nm excitation and 460 nm emission (Carl Zeiss epifluorescence microscope) [36]

Wound Healing Topicals: Emerging Options. ClevelandClinic.org